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quantitect multiplex pcr kit  (Qiagen)


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    Qiagen quantitect multiplex pcr kit
    Quantitect Multiplex Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantitect+multiplex+pcr+kit/QuantiTect+Multiplex+PCR+Kit+x+%E2%80%8B%E2%80%8B200u/10__1038_slash_s41598___026___63878___1-152-33-37
    Average 95 stars, based on 313 article reviews
    quantitect multiplex pcr kit - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Genotyping:

    Article Title: The association between the GNB3 rs5443 C/C genotype and obesity phenotypes in Taiwanese individuals
    Article Snippet: The DNA from buccal samples was extracted using the QIAamp DNA MicroKit (QIAGEN, German) according to manufacturer’s instructions. .. GNB3 rs5443 genotyping was performed using the TaqMan SNP genotyping assay (Thermo-Fisher, USA) and QuantiTect Multiplex PCR Kit (QIAGEN, German). ..

    Article Title: Allele-specific GNB3 modulation of APOE ε4 memory effects
    Article Snippet: .. GNB3 C825T genotyping Genotyping of the GNB3 C825T polymorphism (rs5443) was performed on residual genomic DNA samples from APOE phenotyping using TaqMan SNP Genotyping Assays (Thermo Fisher Scientific, USA) in conjunction with the QuantiTect Multiplex PCR Kit (QIAGEN, Germany). ..

    TaqMan SNP Genotyping Assay:

    Article Title: The association between the GNB3 rs5443 C/C genotype and obesity phenotypes in Taiwanese individuals
    Article Snippet: The DNA from buccal samples was extracted using the QIAamp DNA MicroKit (QIAGEN, German) according to manufacturer’s instructions. .. GNB3 rs5443 genotyping was performed using the TaqMan SNP genotyping assay (Thermo-Fisher, USA) and QuantiTect Multiplex PCR Kit (QIAGEN, German). ..

    Multiplex Assay:

    Article Title: The association between the GNB3 rs5443 C/C genotype and obesity phenotypes in Taiwanese individuals
    Article Snippet: The DNA from buccal samples was extracted using the QIAamp DNA MicroKit (QIAGEN, German) according to manufacturer’s instructions. .. GNB3 rs5443 genotyping was performed using the TaqMan SNP genotyping assay (Thermo-Fisher, USA) and QuantiTect Multiplex PCR Kit (QIAGEN, German). ..

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described , .

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes.
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described7,35.

    Article Title: Genetic/epigenetic RNA dysregulation in type 2 diabetes mellitus complicated with ischemic heart disease
    Article Snippet: .. Reference gene stability and assay performance are summarized in . mRNA targets ( FZD5 , GTF2I ) were quantified using gene-specific primer assays in combination with the QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany, cat. no. 249900; assay IDs QT00200886 and QT01677305), with GAPDH and ACTB serving as internal references. .. For miRNA measurements, hsa-miR-1976 was amplified with the miRCURY LNA SYBR Green PCR Kit (Qiagen, cat. no. 339345) and the corresponding assay (Cat. No. 339350; ID: ZP00000388), and expression was normalized to SNORD44 .

    Article Title: Extracellular matrix proteins refine microenvironments for pancreatic organogenesis from induced pluripotent stem cell differentiation
    Article Snippet: .. TaqMan qRT-PCR was performed using 100 or 200 ng RNA per reaction and QuantiTect Multiplex PCR kit (QIAGEN) on a CFX Connect Real-Time PCR system (Bio-Rad). ..

    Article Title: Nucleotide analogs for efficient DNA and RNA amplification
    Article Snippet: .. Set 1 and Set 2 primers were tested in PCR with QuantiTect® multiplex PCR kit (Qiagen, Cat #204543), Takara® One Step PrimeScriptTM III RT-PCR Kit (Takara Bio, RR600B), and TaqPath® 1-Step RT-qPCR Master Mix (ThermoFisher, A15299) in the “No Template Control” (NTC) reactions, where primer dimers are the only likely products (Table 3-2). ..

    Article Title: Microbial source tracking using metagenomic analysis from Cochin Estuary
    Article Snippet: .. Indexing PCR was performed with QuantiTect MultiPlex PCR kit (Qiagen, Germany) using index primers, adapters, and unique barcode sequences. .. The cycling conditions comprised of 95°C-15 minutes, succeeded by 30 cycles of 95°C-30 sec, 63°C-45 sec, and 72°C-90 sec. After PCR, subsequent quantification of the amplified products was performed in the Broad Range Qubit System (Life Technologies, CA, USA) ( ).

    Article Title: Allele-specific GNB3 modulation of APOE ε4 memory effects
    Article Snippet: .. GNB3 C825T genotyping Genotyping of the GNB3 C825T polymorphism (rs5443) was performed on residual genomic DNA samples from APOE phenotyping using TaqMan SNP Genotyping Assays (Thermo Fisher Scientific, USA) in conjunction with the QuantiTect Multiplex PCR Kit (QIAGEN, Germany). ..

    Polymerase Chain Reaction:

    Article Title: The association between the GNB3 rs5443 C/C genotype and obesity phenotypes in Taiwanese individuals
    Article Snippet: The DNA from buccal samples was extracted using the QIAamp DNA MicroKit (QIAGEN, German) according to manufacturer’s instructions. .. GNB3 rs5443 genotyping was performed using the TaqMan SNP genotyping assay (Thermo-Fisher, USA) and QuantiTect Multiplex PCR Kit (QIAGEN, German). ..

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described , .

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes.
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described7,35.

    Article Title: Genetic/epigenetic RNA dysregulation in type 2 diabetes mellitus complicated with ischemic heart disease
    Article Snippet: .. Reference gene stability and assay performance are summarized in . mRNA targets ( FZD5 , GTF2I ) were quantified using gene-specific primer assays in combination with the QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany, cat. no. 249900; assay IDs QT00200886 and QT01677305), with GAPDH and ACTB serving as internal references. .. For miRNA measurements, hsa-miR-1976 was amplified with the miRCURY LNA SYBR Green PCR Kit (Qiagen, cat. no. 339345) and the corresponding assay (Cat. No. 339350; ID: ZP00000388), and expression was normalized to SNORD44 .

    Article Title: Extracellular matrix proteins refine microenvironments for pancreatic organogenesis from induced pluripotent stem cell differentiation
    Article Snippet: .. TaqMan qRT-PCR was performed using 100 or 200 ng RNA per reaction and QuantiTect Multiplex PCR kit (QIAGEN) on a CFX Connect Real-Time PCR system (Bio-Rad). ..

    Article Title: Nucleotide analogs for efficient DNA and RNA amplification
    Article Snippet: .. Set 1 and Set 2 primers were tested in PCR with QuantiTect® multiplex PCR kit (Qiagen, Cat #204543), Takara® One Step PrimeScriptTM III RT-PCR Kit (Takara Bio, RR600B), and TaqPath® 1-Step RT-qPCR Master Mix (ThermoFisher, A15299) in the “No Template Control” (NTC) reactions, where primer dimers are the only likely products (Table 3-2). ..

    Article Title: Microbial source tracking using metagenomic analysis from Cochin Estuary
    Article Snippet: .. Indexing PCR was performed with QuantiTect MultiPlex PCR kit (Qiagen, Germany) using index primers, adapters, and unique barcode sequences. .. The cycling conditions comprised of 95°C-15 minutes, succeeded by 30 cycles of 95°C-30 sec, 63°C-45 sec, and 72°C-90 sec. After PCR, subsequent quantification of the amplified products was performed in the Broad Range Qubit System (Life Technologies, CA, USA) ( ).

    Article Title: Allele-specific GNB3 modulation of APOE ε4 memory effects
    Article Snippet: .. GNB3 C825T genotyping Genotyping of the GNB3 C825T polymorphism (rs5443) was performed on residual genomic DNA samples from APOE phenotyping using TaqMan SNP Genotyping Assays (Thermo Fisher Scientific, USA) in conjunction with the QuantiTect Multiplex PCR Kit (QIAGEN, Germany). ..

    cDNA Synthesis:

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described , .

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes.
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described7,35.

    Real-time Polymerase Chain Reaction:

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described , .

    Article Title: Functional significance of commonly regulated genes in mechanically and chemically induced retinal ganglion cell death in rat eyes.
    Article Snippet: .. For some experiments, the PrimeScript RT Reagent Kit (Takara, Shiga, Japan) and QuantiTect Multiplex PCR Kit (Qiagen, Hilden, Germany) were used for cDNA synthesis and real-time PCR, respectively. .. For real-time PCR, a predesigned primer-probe mixture for Gapdh (Applied Biosystems, Waltham, MA) or Nefl (Sigma-Aldrich, St. Louis, MO, USA) was used as previously described7,35.

    Article Title: Extracellular matrix proteins refine microenvironments for pancreatic organogenesis from induced pluripotent stem cell differentiation
    Article Snippet: .. TaqMan qRT-PCR was performed using 100 or 200 ng RNA per reaction and QuantiTect Multiplex PCR kit (QIAGEN) on a CFX Connect Real-Time PCR system (Bio-Rad). ..

    Quantitative RT-PCR:

    Article Title: Extracellular matrix proteins refine microenvironments for pancreatic organogenesis from induced pluripotent stem cell differentiation
    Article Snippet: .. TaqMan qRT-PCR was performed using 100 or 200 ng RNA per reaction and QuantiTect Multiplex PCR kit (QIAGEN) on a CFX Connect Real-Time PCR system (Bio-Rad). ..

    Article Title: Nucleotide analogs for efficient DNA and RNA amplification
    Article Snippet: .. Set 1 and Set 2 primers were tested in PCR with QuantiTect® multiplex PCR kit (Qiagen, Cat #204543), Takara® One Step PrimeScriptTM III RT-PCR Kit (Takara Bio, RR600B), and TaqPath® 1-Step RT-qPCR Master Mix (ThermoFisher, A15299) in the “No Template Control” (NTC) reactions, where primer dimers are the only likely products (Table 3-2). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Nucleotide analogs for efficient DNA and RNA amplification
    Article Snippet: .. Set 1 and Set 2 primers were tested in PCR with QuantiTect® multiplex PCR kit (Qiagen, Cat #204543), Takara® One Step PrimeScriptTM III RT-PCR Kit (Takara Bio, RR600B), and TaqPath® 1-Step RT-qPCR Master Mix (ThermoFisher, A15299) in the “No Template Control” (NTC) reactions, where primer dimers are the only likely products (Table 3-2). ..

    Control:

    Article Title: Nucleotide analogs for efficient DNA and RNA amplification
    Article Snippet: .. Set 1 and Set 2 primers were tested in PCR with QuantiTect® multiplex PCR kit (Qiagen, Cat #204543), Takara® One Step PrimeScriptTM III RT-PCR Kit (Takara Bio, RR600B), and TaqPath® 1-Step RT-qPCR Master Mix (ThermoFisher, A15299) in the “No Template Control” (NTC) reactions, where primer dimers are the only likely products (Table 3-2). ..



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